A Simple Western Size™ assay for the detection of AXL protein expression in breast cancer tissue was developed to detect the AXL receptor tyrosine kinase in tissue lysates. The AXL receptor tyrosine kinase is expressed in a variety of cancers and has been revealed as the most highly expressed gene in preclinical models with acquired resistance to targeted therapy. Upregulation and/or activation of AXL are also shown to be predictive of lack of response to ErbB family receptor-targeted inhibitors, such as Her2-targeted agents.
